Stage III / 2019

STAGE III / 2019In vitro demonstration and evaluation of the functionality of 3D stem cells-matrix systems in the treatment of complex lesions of oral tissue

Activities

III.1. Screening of cell differentiation and adhesion capacity and establishing the optimal cultivation parameters – Part II

III.2. Evaluation of DPSC secreted factors on the synthesis of new ECM.

III.3. Demonstration of 3D stem cell-matrix system functionality and utility for treatment of complex oral tissue conditions

III.4. Dissemination of results, webpage and reporting

III.5. Documentation for patent demand

III.6. Project management

 

Stage III results

  • Optimized experimental models for structural analysis of 3D cell culture systems
  • Lab technology for obtaining  matrix-stem cells 3D systems
  • Functional variants of  matrix-stem cells 3D systems

Stage III summary

 

Dissemination of results

Article

Seciu AM., Craciunescu O., Stanciuc AM., Zarnescu O. 2019. Tailored biomaterials for therapeutic strategies applied in periodontal tissue engineering. Stem Cells and Development. vol. 28, no. 15, 963–973. [Link]

Patent application

OSIM no. A/00454 / 26.07.2019. Zarnescu O., Craciunescu O., Seciu AM., Moldovan L., Gaspar-Pintiliescu A., Stanciuc AM., Stefan LM. Biopolymeric composite hydrogel for treatment of periodontal disease and process for obtaining thereof.

Conferences

Zarnescu O., Seciu AM., Craciunescu O., Moldovan L. Multilineage differentiation capacity of human dental pulp stem cells cryopreserved for regenerative medicine applications. European Biotechnology Congress, April 11-13, 2019, Valencia, Spain. Published in J. Biotechnology, 2019, 305, Supplement, p. S85.  [Link]

Seciu AM., Craciunescu O., Stan L., Zarnescu O. 2019. Anti-inflammatory activity of biomaterials intended for periodontal disease treatment. PRIOCHEM 15th edition, 30 oct – 1 nov 2019, Bucharest, Romania. Published in MDPI Proceedings 2019, 29 (1), p. 66 [Link]

 
Other results
  • Lab technology for the preparation of 3D cellular systems by cultivation of spheroids from dental pulp stem cells in a biomimetic matrix from components of extracellular matrix.
  • 3D functional systems of scaffold matrices for the spheroids from dental pulp stem cells with anti-inflammatory and regenerative properties for increasing the cell survival after transplantation.