Stage II / 2018

STAGE II / 2018: Development and testing of new 3D cell culture systems of DPSC embedded in matrix equivalents

Activities

II.1. Evaluation of specific markers using optimized detection methods – Part II

II.2. Preparation and screening of 3D biomimetic oral ECM equivalents for DPSC embedding – Part II

II.3. Obtaining of DPSC spheroids

II.4. Development of DPSC- matrix 3D systems

II.5. Screening of cell differentiation and adhesion capacity and establishing the optimal cultivation parameters – Part I

II.6. Dissemination of results, webpage and reporting

II.7. Project management

        

Stage II results

  • Set of optimal parameters for cultivation of DPSC as 3D spheroids
  • Set of experimental variants of 3D stem cell systems
  • Experimental technology for obtaining variants of  matrix-stem cells 3D systems
  • Set of experimental variants of  matrix-stem cells 3D systems
  • Optimized methods for structural analysis of 3D cell culture systems

Stage II Summary

 

Dissemination of results

Article

  • Craciunescu O., Gaspar-Pintiliescu A., Seciu A-M., Moldovan L., Zarnescu O. 2019. Structure and cytotoxicity of a porous biomimetic material for oral tissue wound healing. Physica Status Solidi(a)-Applications and Materials Science, [Link].

Book chapter

  • Seciu AM., Craciunescu O., Zarnescu O. 2018. Advanced regenerative techniques based on dental pulp stem cells for the treatment of periodontal disease. In: Periodontology, IntechOpen   Doi: 10.5772/intechopen.78048. [Link]

International conferences

  • Zarnescu O., Craciunescu O., Seciu AM., Stanciuc AM., Moldovan L. Collagen-chondroitin 4 sulfate-fibronectin scaffold: Characterization and in vitro biocompatibility. European Biotechnology Congress, April 26-28, 2018, Athens, Greece. Journal of Biotechnology 280S (2018) S91 [Link]
  • Craciunescu O., Seciu AM., Gaspar A., Moldovan L. Zarnescu O. Microscopic characterization and in vitro biocompatibility of a biomimetic scaffold for regeneration of oral tissue lesions. The European Materials Research Society (E-MRS) Spring Meeting, June 18-22, 2018, Strasbourg, France. [Link]
  • Zarnescu O., Seciu AM., Craciunescu O., Moldovan L. Age-related properties of human dental pulp stem cells cryopreserved for regenerative medicine. 29th Annual Meeting of the European Society for Biomaterials (ESB), September 9-13, 2018, Maastricht, Netherlands. ESB Abstract Proceedings
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