Results and project dissemination
Stage I results
- Set of optimal parameters for cryoconservation of stem cells from dental pulp (DPSC)
- Set of optimal parameters for cultivation of DPSC after cryoconservation
- Set of experimental variants of 3D biomimetic matrices
Stage II results
- Set of optimal parameters for cultivation of DPSC as 3D spheroids
- Set of experimental variants of 3D stem cell systems
- Experimental technology for obtaining variants of matrix-stem cells 3D systems
- Set of experimental variants of matrix-stem cells 3D systems
- Optimized methods for structural analysis of 3D cell culture systems
Stage III results
- Optimized experimental models for structural analysis of 3D cell culture systems
- Lab technology for obtaining matrix-stem cells 3D systems
- Functional variants of matrix-stem cells 3D systems
Dissemination of results
Articles
- Craciunescu O., Gaspar-Pintiliescu A., Seciu A-M., Moldovan L., Zarnescu O. 2019. Structure and cytotoxicity of a porous biomimetic material for oral tissue wound healing. Physica Status Solidi(a)-Applications and Materials Science, vol. 206, no. 11, 1970039. [Link]
- Seciu AM., Craciunescu O., Stanciuc AM., Zarnescu O. 2019. Tailored biomaterials for therapeutic strategies applied in periodontal tissue engineering. Stem Cells and Development. vol. 28, no. 15, 963–973. [Link]
Book chapter
- Seciu AM., Craciunescu O., Zarnescu O. 2019. Advanced regenerative techniques based on dental pulp stem cells for the treatment of periodontal disease. In: Periodontology and Dental Implantology, J Manakil, ed. InTechOpen Ltd, London, UK, pp. 129-148. [Link]
Patent application
- OSIM no. A/00454 / 26.07.2019. Zarnescu O., Craciunescu O., Seciu AM., Moldovan L., Gaspar-Pintiliescu A., Stanciuc AM., Stefan LM. Biopolymeric composite hydrogel for treatment of periodontal disease and process for obtaining thereof.
Conferences
- Craciunescu O., Seciu AM., Gaspar A., Moldovan L., Zarnescu O. Development of 3D matrix equivalent for regeneration of complex oral tissue lesions. AMBA (Advanced Materials for Biomedical Applications) 2017, September 27-29 2017, Ghent, Belgium. [Link]
- Zarnescu O., Craciunescu O., Seciu AM., Stanciuc AM., Moldovan L. Collagen-chondroitin 4 sulfate-fibronectin scaffold: Characterization and in vitro biocompatibility. European Biotechnology Congress, April 26-28, 2018, Athens, Greece. Abstract published in Journal of Biotechnology, 2018, 280S, S91 [Link]
- Craciunescu O., Seciu AM., Gaspar A., Moldovan L. Zarnescu O. Microscopic characterization and in vitro biocompatibility of a biomimetic scaffold for regeneration of oral tissue lesions. The European Materials Research Society (E-MRS) Spring Meeting, June 18-22, 2018, Strasbourg, France. [Link]
- Zarnescu O., Seciu AM., Craciunescu O., Moldovan L. Age-related properties of human dental pulp stem cells cryopreserved for regenerative medicine. 29th Annual Meeting of the European Society for Biomaterials (ESB), September 9-13, 2018, Maastricht, Netherlands. ESB Abstract Proceedings.
- Zarnescu O., Seciu AM., Craciunescu O., Moldovan L. Multilineage differentiation capacity of human dental pulp stem cells cryopreserved for regenerative medicine applications. European Biotechnology Congress, April 11-13, 2019, Valencia, Spain. Published in Journal of Biotechnology, 2019, 305, Supplement, p. S85. [Link]
- Seciu AM., Craciunescu O., Stan L., Zarnescu O. 2019. Anti-inflammatory activity of biomaterials intended for periodontal disease treatment. PRIOCHEM 15th edition, 30 oct – 1 nov 2019, Bucharest, Romania. Published in MDPI Proceedings 2019, 29 (1), p. 66 [Link]